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Plant Physiology 68:626-630 (1981)
© 1981 American Society of Plant Biologists

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Isolation and Properties of Ion-Stimulated ATPase Activity Associated with Cauliflower Plasma Membranes 1

Edward J. McMurchie2 and M. Keith Pomeroy3

2 Plant Physiology Unit, Commonwealth Scientific and Industrial Research Organization, Division of Food Research, School of Biological Sciences, Macquarie University, North Ryde, N. S. W. 2113, Australia, 3 Research Branch, Agriculture Canada, Ottawa, Ontario K1A OC6 Canada

The association of K+-stimulated, Mg2+-dependent ATPase activity with plasma membranes from higher plants has been used as a marker for the isolation and purification of a plasma membrane-enriched fraction from cauliflower (Brassica oleraceae L.) buds. Plasma membranes were isolated by differential centrifugation followed by density gradient centrifugation of the microsomal fraction. The degree of purity of plasma membranes was determined by increased sensitivity of Mg2+-ATPase activity to stimulation by K+ and by assay of approximate marker enzymes. In the purified plasma membrane fraction, Mg2+-ATPase activity was stimulated up to 700% by addition of K+. Other monovalent cations also markedly stimulated the enzyme, but only in the presence of the divalent cation Mg2+. Ca2+ was inhibitory to enzyme activity. ATPase was the preferred substrate for hydrolysis, there being little hydrolysis in the presence of ADP, GTP, or p-nitrophenylphosphate. Monovalent cation-stimulated activity was optimum at alkaline pH. Enzyme activity was inhibited nearly 100% by AgNO3 and about 40% by diethylstilbestrol.

1 Contribution 1189 of Chemistry and Biology Research Institute, Agriculture Canada.






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