This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Kagawa, T. Articles by González, E. Search for Related Content PubMed PubMed Citation Articles by Kagawa, T. Articles by González, E. Agricola Articles by Kagawa, T. Articles by González, E.

Articles

Organelle-Specific Isozymes of Citrate Synthase in the Endosperm of Developing Ricinus Seedlings ¹

Elma González

Biochemistry Department, University of Missouri, Columbia, MO 65211, Department of Biology, University of California, Los Angeles, CA 90024

Chromatographic analysis of organelle-associated citrate synthase activity revealed distinct mitochondrial and glyoxysomal forms of the enzyme. The chromatographic elution patterns on hydroxylapatite, carboxymethylcellulose and DEAE-cellulose of citrate synthase from the endosperm of 4.5-day-old castor bean seedlings revealed significant differences for mitochondrial and glyoxysomal activities of the enzyme. The endoplasmic reticulum-associated citrate synthase activity eluted from DEAE-cellulose in a pattern that was identical to that of the glyoxysomal activity. The same kinds of organelle specific isozyme elution patterns were observed with young, developing seedlings. Gibberellic acid-treatment of young seedlings increased total recoverable citrate synthase activity from endosperm tissue but did not modify the organelle specific isozyme relationships.