Plant Physiol. Drug Metab Dispos
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Plant Physiology 68:910-913 (1981)
© 1981 American Society of Plant Biologists

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Articles

Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus vulgaris L. 1

Gerrit-Jan van Holst, Frans M. Klis, Piet J. M. de Wildt, Corinne A. M. Hazenberg, Jopie Buijs and Durk Stegwee

Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands

Sonication of a crude cell organelle fraction from hypocotyl tissue of dark-grown bean seedlings, and from suspension-cultured cells released a hydroxyproline-containing protein. The purification of this protein is described. It was found to be an arabinogalactan protein composed of 90% carbohydrate and 10% protein. The major sugars are galactose, arabinose, and uronic acids, and the major amino acids are hydroxyproline, serine, and alanine. Its molecular weight was estimated at 1.4 x 105 daltons and the isoelectric point at pH 2.3. The molecule is soluble in 5% trichloroacetic acid and can be precipitated with {beta}-galactosyl Yariv antigen. Pulse-chase experiments indicated that it was a secretory protein. The biosynthesis of arabinogalactan proteins is discussed.

1 Supported by the Foundation for Fundamental Biological Research (BION) which is subsidized by the Netherlands Organization for the Advancement of Pure Research Z. W. O.




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A. J. van Hengel, Z. Tadesse, P. Immerzeel, H. Schols, A. van Kammen, and S. C. de Vries
N-Acetylglucosamine and Glucosamine-Containing Arabinogalactan Proteins Control Somatic Embryogenesis
Plant Physiology, April 1, 2001; 125(4): 1880 - 1890.
[Abstract] [Full Text]


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Copyright © 1981 by the American Society of Plant Biologists