Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 68:1289-1293 (1981)
© 1981 American Society of Plant Biologists

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Articles

Demonstration of Auxin Binding to Strawberry Fruit Membranes 1,2

Komaratchi R. Narayanan, Kenneth W. Mudge3 and B. W. Poovaiah4

Department of Horticulture and Landscape Architecture, Washington State University, Pullman, Washington 99164

Presence of specific auxin-binding sites in strawberry fruit (Fragaria ananassa Duch. cv. Ozark Beauty) membranes has been demonstrated. These 1-naphthaleneacetic acid (NAA)-binding sites in the 80,000g to 120,000g fraction of the strawberry fruit membrane were pronase sensitive with an estimated equilibrium dissociation constant for NAA of 1.1 x 10–6 molar. The minimum concentration of NAA required to stimulate strawberry fruit growth was at least one order of magnitude higher than the minimum concentration of NAA required to stimulate corn coleoptile elongation. This was consistent with the higher equilibrium dissociation constant (lower affinity) for auxin binding to strawberry fruit membranes than to corn coleoptiles. Twelve auxin analogs, ranging from very strong to weak auxins, were tested for abilities to stimulate in situ strawberry fruit growth and to bind (displace or compete with NAA) to strawberry fruit membranes. The observed positive correlation (r = 0.74) between the in vitro binding to the 80,000 to 120,000 membrane fraction and the in situ biological activity of these analogs indicated that the NAA-binding sites in strawberry fruit membranes may represent physiologically relevant auxin receptors.

3 Present address: Department of Floriculture and Ornamental Horticulture, Cornell University, Ithaca, NY.

4 To whom reprint requests should be addressed.

1 Supported by National Science Foundation Grant PCM 78-05292.

2 Scientific Paper 5556, College of Agriculture Research Center, Washington State University, Pullman, Project 0321.






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Copyright © 1981 by the American Society of Plant Biologists