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Carbamoyl Phosphate Synthetase Activity from the Cotyledons of Developing and Germinating Pea Seeds

Botanical Laboratory, State University of Utrecht, Utrecht, The Netherlands

Carbamoyl phosphate synthetase activity was measured in partially purified extracts from cotyledons of developing and germinating seeds of Pisum sativum L. Some properties of the enzyme were established. During cotyledon development, the activity initially increased sharply but decreased during further development. The activity from germinating seeds was only one-tenth of the maximum activity at an early developmental phase. The results are discussed in relation to pea seed development and germination.