Plant Physiology 69:150-154 (1982)
© 1982 American Society of Plant Biologists
Articles
In Vitro Synthesis and Processing of Wheat  -Amylase 1
TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS
Rebecca S. Boston,
Timothy J. Miller2,
Janet E. Mertz and
Richard R. Burgess
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706
Wheat (Triticum aestivum) RNA was used to program synthesis of the  -amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as -amylase by immunoprecipitation with rabbit anti--amylase antiserum raised against the purified wheat protein and by its co-migration with authentic -amylase on sodium dodecyl sulfate polyacrylamide gels. Synthesis of -amylase was dependent upon injection of RNA extracted from gibberellic acid-induced aleurone layers from wheat. The amount of -amylase produced was proportional to the amount of RNA injected and reached a plateau within 4 hours after injection. When the same RNA was translated in a wheat germ cell-free translation system, a 43,000-dalton protein was produced. Addition of dog pancreas microsomal membranes to the wheat germ translation system resulted in processing of the -amylase protein to a form which co-migrated with authentic -amylase purified from malted wheat and with the protein synthesized in oocytes.
2 Present address: Armos Corp., 180 Kimball Way, So. San Francisco, CA 94080.
1 Supported by Public Health Service Grants CA-07175, CA-22443, CA-23076, and an American Cancer Society Grant PF-1678 to T. J. M.
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