This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sutton, B. C. S. Articles by Shaw, M. Search for Related Content PubMed PubMed Citation Articles by Sutton, B. C. S. Articles by Shaw, M. Agricola Articles by Sutton, B. C. S. Articles by Shaw, M.

Articles

Changes in Two Ribonuclease Isozymes During Rust Infection of Flax Cotyledons ¹

The University of British Columbia, 2075 Westbrook Place, Vancouver, British Columbia, Canada V6T 1W5

A ribonuclease fraction previously purified from flax by gel filtration was further resolved into two components by hydroxyl apatite chromatography. These were homogeneous with respect to electrophoresis and isoelectric focusing. Both enzymes are of RNase I type but differ in substrate specificity, kinetic properties, pH response, and isoelectric point.

The two RNase isozymes show consistent properties when extracted from variety Bison (susceptible) or variety Bombay (resistant) with or without infection with race 3 of flax rust. The relative amounts of these isozymes change markedly during infection. These observations provide an explanation for the apparent qualitative changes in RNase noted previously. Differences between susceptible and resistant reactions in the early stages of disease are discussed.

This article has been cited by other articles:

				M. A. Ayliffe, J. K. Roberts, H. J. Mitchell, R. Zhang, G. J. Lawrence, J. G. Ellis, and T. J. Pryor A Plant Gene Up-Regulated at Rust Infection Sites Plant Physiology, May 1, 2002; 129(1): 169 - 180. [Abstract] [Full Text] [PDF]