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Characteristics of Teratomas Regenerated in Vitro from Octopine-Type Crown Gall

United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, Cell Culture and Nitrogen Fixation Laboratory, Beltsville, Maryland 20705

Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. Detached leaves from T5 shoots also synthesized octopine. These results establish that shoots from this octopine-type tumor contain transformed cells and are true crown-gall teratomas.

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