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Plant Physiology 69:54-57 (1982)
© 1982 American Society of Plant Biologists

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Measuring Chlorophyll a and 14C-Labeled Photosynthate in Aquatic Angiosperms by the Use of a Tissue Solubilizer 1

Sven Beer2, Arthur J. Stewart3 and Robert G. Wetzel

W. K. Kellogg Biological Station and Department of Botany and Plant Pathology, Michigan State University, Hickory Corners, Michigan 49060

A compound that quantitatively correlated with chlorophyll a could be measured fluorometrically in the extracts of leaves of three aquatic angiosperms (Myriophyllum heterophyllum Michx., Potamogeton crispus L., Elodea canadensis Michx.) treated with the tissue solubilizer BTS-450. Fluorescent characteristics of the solubilized plant tissues were stable for several weeks in the dark at temperatures up to 60°C but rapidly degraded in sunlight or when acidified. 14C-Labeled photosynthate, which had been fixed by leaf discs during 1- to 10-hour exposure to H14CO3, was also readily extracted by the tissue solubilizer. Solubilizer extraction can, therefore, be used to determine both chlorophyll a content and 14C incorporation rates in the same leaf sample. The method is practical, because no grinding is required, the fluorescent characteristics of the extracts are stable, and analyses can be performed with very little plant material (about 3 milligrams).

2 Present address: Department of Botany, Tel Aviv University, Ramat Aviv 69978, Israel.

3 Present address: Environmental Sciences Division, Oak Ridge National Laboratory, P. O. Box X, Oak Ridge, TN 37830.

1 Supported by subventions of the United States Department of Energy (EY-76-S-02-1599, C00-1599-197) and the National Science Foundation (OCE-76-84264). Contribution No. 460, W. K. Kellogg Biological Station, Michigan State University, Hickory Corners, MI 49060.






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Copyright © 1982 by the American Society of Plant Biologists