This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Skokut, T. A. Articles by McKay, R. A. Search for Related Content PubMed PubMed Citation Articles by Skokut, T. A. Articles by McKay, R. A. Agricola Articles by Skokut, T. A. Articles by McKay, R. A.

Articles

¹⁵N- and [¹³C]NMR Determination of Utilization of Glycine for Synthesis of Storage Protein in the Presence of Glutamine in Developing Cotyledons of Soybean

Department of Biology, Washington University, St. Louis, Missouri 63130, Physical Sciences Center, Monsanto Co., St. Louis, Missouri 63166

Solid-state ¹⁵N- and [¹³C] NMR have been used to measure quantitatively the utilization of glycine in the presence of glutamine for the synthesis of storage protein in immature cotyledons of soybean (Glycine max L. cv. Elf) in culture. The presence of an equal molar amount of glycine in the medium causes a decrease in the use of glutamine-amide nitrogen. Glycine nitrogen is incorporated extensively into peptide bonds (in amounts greater than what would be expected if it appeared solely in glycine residues), but is used sparingly for synthesis of histidine ring residues, guanidino nitrogen residues of arginine, and lysine residues. The modest use of glycine carbon in protein synthesis does not parallel the use of glycine nitrogen.