Plant Physiol.
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Plant Physiology 69:338-340 (1982)
© 1982 American Society of Plant Biologists

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Articles

Auxin- and Ethylene-Induced Changes in the Population of Translatable Messenger RNA in Basal Sections and Intact Soybean Hypocotyl 1

Linda L. Zurfluh and Tom J. Guilfoyle

Department of Botany, University of Minnesota, St. Paul, Minnesota 55108

In vitro translation products of polyadenylated RNA from untreated and auxin-treated basal sections of soybean (Glycine max var. Wayne) hypocotyl were analyzed by two-dimensional polyacrylamide gel electrophoresis. Within one hour of 2,4-dichlorophenoxyacetic acid treatment, the translatable messenger RNAs for at least twelve in vitro translation products are modulated upward. In vitro translation products of polyadenylated RNA from untreated, auxin-treated and Ethephon-treated intact soybean hypocotyl were also analyzed. Within two hours of treatment with either 2,4-dichlorophenoxyacetic acid or Ethephon, the translatable messenger RNAs for a group of high molecular weight in vitro translation products are modulated upward. There is a particular set of translatable messenger RNA, encoding in vitro translation products in the 24,000 to 32,000 molecular weight range, that is specifically modulated upward by auxin treatment in intact soybean hypocotyl and in hypocotyl sections.


1 Supported by United States Public Health Service Research Grant GM24096.




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A. B. BLEECKER, M. A. ESTELLE, C. SOMERVILLE, and H. KENDE
Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana
Science, August 26, 1988; 241(4869): 1086 - 1089.
[Abstract] [PDF]




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Copyright © 1982 by the American Society of Plant Biologists