Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 69:442-447 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kow, Y. W.
Right arrow Articles by Gibbs, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kow, Y. W.
Right arrow Articles by Gibbs, M.
Agricola
Right arrow Articles by Kow, Y. W.
Right arrow Articles by Gibbs, M.
Articles

Chloroplast Respiration 1

A MEANS OF SUPPLYING OXIDIZED PYRIDINE NUCLEOTIDE FOR DARK CHLOROPLASTIC METABOLISM

Yoke Wah Kow2, David L. Erbes3 and Martin Gibbs

Institute for Photobiology of Cells and Organelles, Brandeis University, Waltham, Massachusetts 02254

A spinach (Spinacia oleracia var. America) chloroplast particle fortified with ferredoxin, fructose-1,6-bisphosphate, or ribose-5-phosphate and NADP has been shown to generate NADPH by the oxidation of glyceraldehyde-3 phosphate to glycerate-3-phosphate (PGA) and to reduce ferredoxin with the NADPH. The resulting reduced ferredoxin can reduce O2 to H2O2, nitrite to ammonia, or protons to H2. Hydrogen production was the result of adding hydrogenase from Chlamydomonas reinhardii to the chloroplast preparation. The predicted stoichiometry of 1 PGA:1 O2 in the absence of and 2 PGA:1 O2 in the presence of catalase was observed indicating H2O2 as the end product of O2 reduction. The predicted stoichiometry of 3 PGA:1 nitrite:1 ammonia was also observed. A scheme is presented to account for a sustained generation of NADP and ATP necessary for the dissimilation of starch in the darkened chloroplast. The unifying term chloroplast respiration is introduced to account for those reactions in which reduced ferredoxin interacts with physiological acceptors other than NADP or nitrite, hydrogen, or O2 respiration when nitrite, protons, or O2 is the ultimate electron acceptor.

2 Present address: Department of Biochemistry, University of Wisconsin, Madison, WI 53706.

3 Present address: Biochemicals Department, DuPont Experimental Station, Wilmington, DE 19898.

1 This research was supported by National Science Foundation PCM-7682157 and Department of Energy DE-AC02-76-ER03231.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists