Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 69:682-686 (1982)
© 1982 American Society of Plant Biologists

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Articles

Bud Induction with Cytokinin 1

A LOCAL RESPONSE TO LOCAL APPLICATION

W. Scott Grayburn2, Paul B. Green3 and Guy Steucek

Department of Biological Sciences, Stanford University, Stanford, California 94305

A portion of the surface of detached Graptopetalum paraquayense E. Walther leaves can be used to assay small amounts of reagents in lanolin for their ability to induce shoots only at the site of application. The cytokinins benzyladenine, kinetin, and 6-({gamma},{gamma}-dimethylallylamino)purine (DMAAP) were tested, and DMAAP was most effective in bud induction at concentrations below 1%. The higher the hormone concentration, the sooner the appearance of leaf primordia and the higher the ultimate yield of buds. Leaves treated with DMAAP for 2 days developed buds as rapidly as those with longer treatments.

2 Present address: University of Washington, A.J.-10 Botany, Seattle, WA 98195.

3 To whom reprint requests should be addressed.

1 Supported by National Science Foundation Grant PCM78-83244-A2.




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