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Plant Physiology 69:717-723 (1982)
© 1982 American Society of Plant Biologists

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Articles

Dissociation, Reassociation, and Purification of Plastid and Cytosolic Phosphoglucose Isomerase Isozymes 1

Norman F. Weeden and Leslie D. Gottlieb

Department of Plant and Soil Biology, University of California, Berkeley, California 94720, Department of Genetics, University of California, Davis, California 95616

The plastid and cytosolic isozymes of the dimeric enzyme phosphoglucose isomerase (EC 5.3.1.9) from spinach (Spinacia oleracea) and cauliflower (Brassica oleracea) were purified to apparent homogeneity. The isozymes from sunflower (Helianthus annuus) and Clarkia xantiana were partially purified. When subunits from two electrophoretically distinguishable cytosolic isozymes, either from the same or from different species, were dissociated and allowed to reassociate in each other's presence, an active hybrid enzyme, consisting of one subunit of each type, was formed in addition to the two original homodimers. Active hybrid enzymes were also formed by dissociation and reassociation of plastid isozymes. Hybrid molecules were not produced between the plastid and cytosolic subunits, suggesting that they are not able to bind with each other. Additional differences between the plastid and cytosolic isozymes are described.

1 Supported in part by a National Research Service Award, Grant No. 5 T32 GM07467 (to N. F. W.), and National Science Foundation Grant DEB 79-22196 to (L. D. G.). This paper represents part of the PhD dissertation of N. F. W.




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Copyright © 1982 by the American Society of Plant Biologists