Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 69:1040-1045 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uribe, E. G.
Right arrow Articles by Stark, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uribe, E. G.
Right arrow Articles by Stark, B.
Agricola
Right arrow Articles by Uribe, E. G.
Right arrow Articles by Stark, B.
Articles

Inhibition of Photosynthetic Energy Conversion by Cupric Ion 1

EVIDENCE FOR Cu2+-COUPLING FACTOR 1 INTERACTION

Ernest G. Uribe and Benjamin Stark2

Department of Botany, Washington State University, Pullman, Washington 99164

This study describes a specific Cu2+ and light-dependent inhibition of spinach (Spinacia oleracea L.) chloroplast reactions involving coupling factor 1 function. A primary effect is an inhibition of photophosphorylation induced by illumination of Class II chloroplasts with micromolar Cu2+ and pyocyanine in the absence of ADP, Mg2+, and HPO42–. The inhibition, which is dependent on free Cu2+ as indicated by protection by ethylene diamine tetraacetic acid and dithiothreitol, requires illumination (electron flow) for establishment of the specific inhibition to be noted. Protection is also afforded by uncouplers and some partial protection is provided by micromolar concentrations of ADP and ATP. The data strongly suggest that Cu2+ causes an O2-independent oxidation of sulfhydryl groups on coupling factor 1, which are essential to catalytic function. This conclusion is supported by the reduction of energy-dependent 3H-N-ethylmaleimide labeling of the {gamma} subunit of coupling factor 1 by the Cu2+-light pretreatment.

2 Present address: Department of Zoology, Indiana University, Bloomington, IN 47401.

1 Supported by Grant No. BMS 75-00433 to E. Uribe from the National Science Foundation.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
E. Patsikka, M. Kairavuo, F. Sersen, E.-M. Aro, and E. Tyystjarvi
Excess Copper Predisposes Photosystem II to Photoinhibition in Vivo by Outcompeting Iron and Causing Decrease in Leaf Chlorophyll
Plant Physiology, July 1, 2002; 129(3): 1359 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Pätsikkä, E.-M. Aro, and E. Tyystjärvi
Increase in the Quantum Yield of Photoinhibition Contributes to Copper Toxicity in Vivo
Plant Physiology, June 1, 1998; 117(2): 619 - 627.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists