Plant Physiol. Drug Metab Dispos
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Plant Physiology 69:1121-1127 (1982)
© 1982 American Society of Plant Biologists

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Articles

Cyanide-Insensitive Respiration in Relation to Growth of a Low-Temperature Basidiomycete

David B. Chalkley1 and Roy L. Millar

Department of Plant Pathology, Cornell University, Ithaca, New York

The cyanogenic low-temperature basidiomycete (Coprinus psychromorbidus Redhead and Traquair), unlike other cyanide-tolerant fungi, does not detoxify cyanide via formamide hydro-lyase. Instead, tolerance apparently depends on cyanide-insensitive respiration involving activity of the mitochondrial alternative oxidase. Respiration and growth of young mycelium that lacks alternative oxidase activity are blocked both by cyanide and 1 µM antimycin. When activity of the alternative oxidase is elicited in young mycelium by 0.05 mM cyanide, subsequent treatment with antimycin stimulates respiration and fails to halt growth. Older mycelium becomes tolerant coincidentally with the release of cyanide by the mycelium. Tolerant older mycelium in medium containing 0.05 to 1.0 µM antimycin grows at 30 to 45% of the control rate. Cyanide- and antimycin-tolerant growth and respiration are blocked by salicyl hydroxamic acid, an inhibitor of the alternative oxidase, and by rotenone, which inhibits ATP synthesis at site I.

1 Throughout the duration of this work, the senior author was supported by a National Science Foundation Graduate Fellowship for which he is very thankful. Present address:% Peace Corps, Banjul, The Gambia.






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Copyright © 1982 by the American Society of Plant Biologists