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Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba¹

Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351, Japan, USDA/SEA, University of Illinois, Urbana, Illinois 61801, Department of Botany, University of Illinois, Urbana, Illinois 61801, Department of Agronomy, University of Illinois, Urbana, Illinois 61801, Department of Physiology and Biophysics, University of Illinois, Urbana, Illinois 61801

The effects of cations and abscisic acid on chloroplast activity in guard cells of Vicia faba were investigated by analysis of the transient of chlorophyll a fluorescence. When epidermal strips containing guard cells as the only living cells were incubated in water and illuminated with strong light, chlorophyll a fluorescence rose rapidly to a high intensity and then declined slowly to a stationary level. The rate of this decline was enhanced by K⁺ or Na⁺, and the effect of these cations was greater when added with phosphate than with chloride as the anion. Ca²⁺ suppressed the enhancement by Na⁺ and, to a lesser extent, that by K⁺. Abscisic acid also suppressed the enhancement by K⁺ and Na⁺. Since the fluorescence decline reflects the increase of intrathylakoid H⁺ concentration necessary for photophosphorylation, the acceleration of the decline by K⁺ (or Na⁺ in the absence of Ca²⁺) implicates chloroplast activity in ion accumulation by guard cells in the light. The differential effects of phosphate and chloride suggest that chloroplast activity may be involved in malate formation in guard cells in the light.

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