Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 69:1278-1280 (1982)
© 1982 American Society of Plant Biologists

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Articles

Aggregation of Plant Protoplasts by Artificial Lipid Vesicles

Hirofumi Uchimiya, Naomi Kudo, Toshifumi Ohgawara and Hiroshi Harada

Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan

Sonicated unilamellar lipid vesicles, consisting of egg lecithin, stearylamine, and cholesterol in 7:2:1 molar ratios, promoted the aggregation of tobacco (Nicotiana glutinosa) protoplasts with the aid of mono- or divalent cations.

A reaction mixture containing liposomes (0.4 micromoles lipid per milliliter), 50 millimolar CaCl2, 0.5 molar mannitol, and 5 x 105 protoplasts per milliliter resulted in approximately 25% protoplast aggregation. To achieve the maximum protoplast aggregation, 1.6 x 108 liposomes per protoplast per hour would be required.

The kind of liposomes effective in protoplast aggregation were positively charged, small-size vesicles which were obtained either by 60-minute sonication or by membrane filtration in conjunction with sonication.






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Copyright © 1982 by the American Society of Plant Biologists