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Plant Physiology 69:1320-1325 (1982)
© 1982 American Society of Plant Biologists

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Vacuoles from Sugarcane Suspension Cultures 1

II. CHARACTERIZATION OF SUGAR UPTAKE

Margaret Thom, Ewald Komor2 and Andrew Maretzki

Experiment Station, Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701

Vacuoles, isolated from sugarcane (Saccharum sp.) cells, took up 3-O methylglucose and sucrose and the evidence suggests specific transport systems for these sugars. There was no evidence of sugar efflux from preloaded vacuoles. Vacuoles in situ accumulated 3-O methylglucose, sucrose, glucose, and fructose, as shown by incubation of protoplasts with labeled sugar and subsequent analysis of vacuolar and cytoplasmic radio-activity. During the initial minutes of incubation, the amount and concentration of labeled sugar was higher in the cytoplasm than in the vacuole, but subsequently there was active uptake and accumulation into the vacuole. The rate of hexose transfer into the vacuole in situ approached that of hexose uptake by isolated vacuoles; however, the rate of sucrose uptake by isolated vacuoles was below the in situ rate. The site of sucrose synthesis was in the cytoplasm.

2 Present address: Botanisches Institut der Universität Bayreuth, Bayreuth, West Germany.

1 Supported in part by a grant from the Deutsche Forschungsgemeinschaft (to E.K.) and by a grant from the Governor's Agriculture Coordinating Committee, State of Hawaii (to A.M.). Published with the approval of the Director as Paper No. 520 in the Journal Series of the Experimental Station, Hawaiian Sugar Planters' Association.




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S. Aluri and M. Buttner
Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering
PNAS, February 13, 2007; 104(7): 2537 - 2542.
[Abstract] [Full Text] [PDF]


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Copyright © 1982 by the American Society of Plant Biologists