Plant Physiol.
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Plant Physiology 70:350-352 (1982)
© 1982 American Society of Plant Biologists

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Articles

Effect of Butanedioic Acid Mono (2,2-Dimethylhydrazide) on the Activity of Membrane-Bound Succinate Dehydrogenase

Raymond M. See1 and Chester L. Foy

Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

Mitochondria isolated from hypocotyls of five-day-old bean (Phaseolus vulgaris L. `Black Valentine') seedlings rapidly oxidized succinate, malate, and NADH. Oxidation rates, respiratory control, and ADP:O ratios obtained with saturating concentrations of all three substrates indicated that the mitochondria were tightly coupled. The mitochondrial preparation was then employed to investigate the respiration-inhibiting effects of butanedioic acid mono (2,2-dimethyl-hydrazide) (daminozide) a plant growth retardant having structural similarity to an endogenous respiratory substrate (succinate). Daminozide markedly inhibited the activity of membrane-bound succinate dehydrogenase. Inhibition was of the competitive type (apparent Ki, 20.2 millimolar) with respect to succinate. Although not excluding other hypotheses, the results support an active role for daminozide in the suppression of respiration as an important metabolic site of its action as a plant growth regulator.

1 Present address: Union Carbide, Agricultural Products Company, Research Triangle Park, NC 27709.






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Copyright © 1982 by the American Society of Plant Biologists