Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 70:353-356 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishimura, M.
Right arrow Articles by Akazawa, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishimura, M.
Right arrow Articles by Akazawa, T.
Agricola
Right arrow Articles by Nishimura, M.
Right arrow Articles by Akazawa, T.
Articles

Developmental Formation of Glutamine Synthetase in Greening Pumpkin Cotyledons and Its Subcellular Localization 1

Mikio Nishimura, Parichart Bhusawang2, Kazimierz Strzalka3 and Takashi Akazawa

Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan

During the germination of pumpkin (Cucurbita sp. Amakuri Nankin) seeds in dark, the activity of glutamine synthetase in cotyledons gradually increased, reaching a maximum at 5 to 6 days. A measurable enhancement (about 4-fold) of the enzyme activity occurred when the seedlings were exposed to continuous illumination from day 4 up to day 8. Glutamine synthetase activity was detectable only in the cytosolic fraction in the etiolated cotyledons, whereas it was found both in the cytosolic and chloroplast fractions in the green cotyledons. The two isoenzymes of glutamine synthetase have been separated by DEAE-cellulose column chromatography of extracts from the green cotyledons. These data indicate that during the greening process the chloroplastic glutamine synthetase is newly synthesized. The roles of cytosolic and chloroplastic glutamine synthetase in germinating pumpkin cotyledons concerning assimilation of NH3 are discussed.

2 Visiting scientist sponsored by the Japan Society for the Promotion of Science (JSPS) (1980). Present address: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 5, Thailand.

3 Visiting scientist sponsored by the Ministry of Education, Science and Culture (Mombusho) (1980). Present address: Institute of Molecular Biology, Jagellonian University, Krakow, Poland.

1 This is paper 58 in the series of "Structure and Function of Chloroplast Proteins." Supported in part by the research grants (No. 511606, 511806) from the Ministry of Education, Science and Culture (Mombusho).






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists