Plant Physiol.
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Plant Physiology 70:406-409 (1982)
© 1982 American Society of Plant Biologists

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Articles

Host-Pathogen Interactions 1

XXI. Extraction of a Heat-Labile Elicitor of Phytoalexin Accumulation from Frozen Soybean Stems

Gary D. Lyon2 and Peter Albersheim3

Department of Chemistry, Campus Box 215, University of Colorado, Boulder, Colorado 80309

An extract of frozen and thawed soybean (Glycine max L. Merr. cv. Wayne) stems is active, in wounded soybean cotyledons, as a heat-labile elicitor of phytoalexins. The elicitor activity of the extract is destroyed by heating to 95°C for 10 minutes. The fraction that contains heat-labile elicitor activity releases heat-stable elicitor-active molecules from purified soybean cell walls. Heat-labile elicitor activity voids a Bio-Gel P-6 column and can be absorbed onto and eluted from a DEAE Sephadex ion exchange column. Using the cotyledon phytoalexin elicitor assay, maximum heatlabile elicitor activity was obtained when soybean stems were extracted with acetate buffer at pH 6.0. Addition of 1 millimolar CaCl2 increased apparent heat-labile elicitor activity. The heat-labile elicitor stimulated maximum phytoalexin accumulation when applied to cotyledons immediately after the cotyledons were cut. Partially purified stem extracts lost heat-labile elicitor activity during storage for several days at 3°C. The possible role of a heat-labile elicitor in stimulation of phytoalexin accumulation by both abiotic and biotic elicitors is discussed.

2 This work was carried out while G. D. L. was on sabbatical leave from the Scottish Crop Research Institute, Invergowrie, Dundee, Scotland.

3 To whom reprint requests should be addressed.

1 Supported in part by the United States Department of Energy (EY-76-S-02-1426).




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C. J. COOKSEY, P. J. GARRATT, J. S. DAHIYA, and R. N. STRANGE
Sucrose: A Constitutive Elicitor of Phytoalexin Synthesis
Science, June 24, 1983; 220(4604): 1398 - 1400.
[Abstract] [PDF]


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Copyright © 1982 by the American Society of Plant Biologists