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Plant Physiology 70:410-413 (1982)
© 1982 American Society of Plant Biologists

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Identification of the Leaf Vacuole as a Major Nitrate Storage Pool 1

Robert C. Granstedt2 and Ray C. Huffaker

Plant Growth Laboratory and Department of Agronomy & Range Science, University of California at Davis, Davis, California 95616

Highly purified vacuoles were isolated from protoplasts derived from green barley (Hordeum vulgare var. Numar) leaves, in order to determine their role as a NO3– storage sink. {alpha}-Mannosidase and acid phosphatase activities were used as markers to identify vacuoles, {alpha}-mannosidase being the more suitable. Nitrate and {alpha}-mannosidase, which were released from vacuoles destroyed during lysis of protoplasts, moved at unequal rates in the density gradient used for vacuole isolation. Purified vacuoles retained less NO3– than {alpha}-mannosidase during a single washing. Empirically determined corrections were used to account for NO3– movement in estimating the percentage of total cellular nitrate found in the vacuole. Vacuoles from plants grown in the presence of NO3– contained 58% of the total cellular NO3– and therefore represent a major NO3– storage pool.

2 Present address: c/o Standard Fruit Co., Apartado Postal #96, La Ceiba, Honduras, Central America.

1 This research was in part made possible because of a research assistantship awarded by the Agronomy and Range Science Dept., University of California of Davis.




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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists