Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 70:414-417 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koch, J. L.
Right arrow Articles by Ammondson, D. B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koch, J. L.
Right arrow Articles by Ammondson, D. B. S.
Agricola
Right arrow Articles by Koch, J. L.
Right arrow Articles by Ammondson, D. B. S.
Articles

Evidence of Singlet Oxygen Participation in the Chlorophyll-Sensitized Photooxidation of Indoleacetic Acid

James L. Koch, Robert M. Oberlander1, Imre A. Tamas2, Janice L. Germain3 and Debra B. Sherman Ammondson4

Biology Department, Ithaca College, Ithaca, New York 14850

Chlorophyll-sensitized photooxidation of indoleacetic acid (IAA)—with chlorophyll extracted from Pisum sativum L. cv. Alaska W.R.—was determined in the presence of deuterium oxide and known quenchers of singlet oxygen (1O2) to explore the involvement of 1O2 in the reaction. O2 uptake was measured in light in a buffered aqueous micellar system containing Triton X-100, KCl, chlorophyll, and IAA. The rate of O2 uptake was zero in darkness. The reaction was stimulated by deuterium oxide and inhibited by sodium azide indicating that 1O2 participated in IAA photooxidation. Both mannitol and superoxide dismutase failed to inhibit O2 uptake suggesting that neither the hydroxyl radical nor the superoxide anion played a significant role in the reaction.

1 Present address: School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104.

2 To whom correspondence should be addressed.

3 Present address: Hospital of Albert Einstein, College of Medicine, Bronx, NY 10461.

4 Undergraduate Research Participant; National Science Foundation Grant SMI 76-83170. Present address: College of Architecture and Urban Studies, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists