Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 70:511-516 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Birnberg, P. R.
Right arrow Articles by Hanson, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Birnberg, P. R.
Right arrow Articles by Hanson, J. B.
Agricola
Right arrow Articles by Birnberg, P. R.
Right arrow Articles by Hanson, J. B.
Articles

Citrate Transport in Corn Mitochondria 1

Paul R. Birnberg, Deborah L. Jayroe and John B. Hanson

Department of Botany, University of Illinois, Urbana, Illinois 61801

Citrate uptake by corn mitochondria (Zea mays L. B73 x Mol9) was investigated by osmotic swelling and [14C]citrate accumulation. Uptake driven by passive influx, ammonium gradients, and respiration was followed. There was no requirement for phosphate and/or malate to secure citrate uptake, although under some conditions these additives were promotive. Inhibition of the phosphate and dicarboxylate carriers did not eliminate citrate uptake. Citratein/malateout exchange occurs, but at a rate too slow to account for observed citrate uptake, and depletion of endogenous malate only reduced citrate uptake by 38%. It was concluded that citrate can be rapidly accumulated by a mechanism other than by exchange for dicarboxylates. The effect of uncoupler on respiration-driven [14C]citrate accumulation, and studies of passive swelling using ionophores and uncouplers indicated that the major avenue of citrate uptake is by H+/citrate co-transport with a pH optimum near 4.5. The in vivo role of this mechanism is not yet understood.

1 Supported by Contract DE-AC02-76EV00790, United States Department of Energy.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
G. Genchi, A. Spagnoletta, A. De Santis, L. Stefanizzi, and F. Palmieri
Purification and Characterization of the Reconstitutively Active Citrate Carrier from Maize Mitochondria
Plant Physiology, July 1, 1999; 120(3): 841 - 848.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists