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Plant Physiology 70:528-531 (1982)
© 1982 American Society of Plant Biologists

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Articles

Uptake of Benzyladenine by Tuber Slices of Jerusalem Artichoke (Helianthus tuberosus L.) over a Wide Concentration Range 1

Subhash C. Minocha and Per Nissen2

Department of Botany and Plant Pathology, University of New Hampshire, Durham, New Hampshire 03824, Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, California 92093

Uptake of the cytokinin benzyladenine by tuber slices of Jerusalem artichoke (Helianthus tuberosus L.) increased with the concentration of benzyladenine in the medium in the range 10–8 to 10–4 molar. The kinetics of uptake could be precisely represented by four phases of a single, multiphasic isotherm. Km and Vmax increased in a regular manner upon transition to higher phases. The fit to multiphasic kinetics was significantly better than the fit to cooperative kinetics or to the sum of four independent Michaells-Menten terms.

2 Present address: Department of Microbiology and Plant Physiology, University of Bergen, N-5000 Bergen, Norway.

1 Supported by New Hampshire Agricultural Experiment Station Grant H-233. Scientific Contribution No. 1114 from New Hampshire Agricultural Experiment Station.






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Copyright © 1982 by the American Society of Plant Biologists