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Uptake of Benzyladenine by Tuber Slices of Jerusalem Artichoke (Helianthus tuberosus L.) over a Wide Concentration Range ¹

Department of Botany and Plant Pathology, University of New Hampshire, Durham, New Hampshire 03824, Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, California 92093

Uptake of the cytokinin benzyladenine by tuber slices of Jerusalem artichoke (Helianthus tuberosus L.) increased with the concentration of benzyladenine in the medium in the range 10^–8 to 10^–4 molar. The kinetics of uptake could be precisely represented by four phases of a single, multiphasic isotherm. K_m and V_max increased in a regular manner upon transition to higher phases. The fit to multiphasic kinetics was significantly better than the fit to cooperative kinetics or to the sum of four independent Michaells-Menten terms.

¹ Supported by New Hampshire Agricultural Experiment Station Grant H-233. Scientific Contribution No. 1114 from New Hampshire Agricultural Experiment Station.