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Plant Physiology 70:728-734 (1982) © 1982 American Society of Plant Biologists Chloroplast Alkaline Fructose 1,6-Bisphosphatase Exists in a Membrane-Bound FormBoyce Thompson Institute, Ithaca, New York 14853 An alkaline fructose 1,6-bisphosphatase activity associated with soybean (Glycine max cv Beeson) chloroplasts appears to be membrane-bound. The pH optimum of the membrane-associated activity corresponds to that found for activity associated with the stroma. Illumination of washed thylakoids results in an increase in alkaline fructose 1,6-bisphosphatase activity in the absence of any added stromal factors. Exposure to pH 8.0 results in a partial release of enzyme activity from the membrane. The activation status of the enzyme does not appear to alter its association with the membrane.
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