This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (65) Citing Articles via Google Scholar Google Scholar Articles by Cohen, J. D. Search for Related Content PubMed PubMed Citation Articles by Cohen, J. D. Agricola Articles by Cohen, J. D.

Articles

Identification and Quantitative Analysis of Indole-3-Acetyl-L-Aspartate from Seeds of Glycine max L. ¹

Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

Indole-3-acetyl-L-aspartate (IAAsp) was isolated from seeds of Glycine max L. cv. Hark and its identity established by its chromatographic performance and its mass spectral fragmentation. Following acid hydrolysis, the aspartate moiety was shown to be the L-enantiomer by reverse phase high performance liquid chromatographic retention time of the bisethyl ester derivatized with 2,3,4,6-tetra-O-acetyl--D-glycopyranosyl isothiocyanate. Isotope dilution analysis using [¹⁴C]IAAsp as internal standard showed that soybean seed contained 10 µmol/kg IAAsp and this accounted for one-half of the total indoleacetic acid of the seed.

¹ Supported by grants from the National Aeronautics and Space Administration (NAGW-97, ORD 25796) and the National Science Foundation (PCM 79-04637) to Professor R. S. Bandurski. Journal article 10227 from the Michigan Agricultural Experiment Station.

This article has been cited by other articles:

				B. Savic, S. Tomic, V. Magnus, K. Gruden, K. Barle, R. Grenkovic, J. Ludwig-Muller, and B. Salopek-Sondi Auxin Amidohydrolases from Brassica rapa Cleave the Alanine Conjugate of Indolepropionic Acid as a Preferable Substrate: A Biochemical and Modeling Approach Plant Cell Physiol., September 1, 2009; 50(9): 1587 - 1599. [Abstract] [Full Text] [PDF]

				J. J. Campanella, A. F. Olajide, V. Magnus, and J. Ludwig-Muller A Novel Auxin Conjugate Hydrolase from Wheat with Substrate Specificity for Longer Side-Chain Auxin Amide Conjugates Plant Physiology, August 1, 2004; 135(4): 2230 - 2240. [Abstract] [Full Text] [PDF]

				A. Jakubowska and S. Kowalczyk The auxin conjugate 1-O-indole-3-acetyl-{beta}-D-glucose is synthesized in immature legume seeds by IAGlc synthase and may be used for modification of some high molecular weight compounds J. Exp. Bot., April 1, 2004; 55(398): 791 - 801. [Abstract] [Full Text] [PDF]

				S. LeClere, R. Tellez, R. A. Rampey, S. P. T. Matsuda, and B. Bartel Characterization of a Family of IAA-Amino Acid Conjugate Hydrolases from Arabidopsis J. Biol. Chem., May 31, 2002; 277(23): 20446 - 20452. [Abstract] [Full Text] [PDF]

				Y. Y. Tam, E. Epstein, and J. Normanly Characterization of Auxin Conjugates in Arabidopsis. Low Steady-State Levels of Indole-3-Acetyl-Aspartate, Indole-3-Acetyl-Glutamate, and Indole-3-Acetyl-Glucose Plant Physiology, June 1, 2000; 123(2): 589 - 596. [Abstract] [Full Text]