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Plant Physiology 70:1014-1018 (1982)
© 1982 American Society of Plant Biologists

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Articles

Site of Synthesis of NADPH

Protochlorophyllide Oxidoreductase in Rye (Secale cereale) 1

W. T. Griffiths and Neil S. Beer

Department of Biochemistry, The Medical School, University of Bristol, Bristol BS8 1TD England

The site of synthesis of the plastid membrane-located enzyme, protochlorophyllide reductase, has been determined. Plastid ribosome-deficient and normal rye (Secale cereale L., cv Rheidol) plants were grown in darkness at 33°C and 22°C, respectively. Extracts from these plants were analyzed for the levels of different ribosomal RNAs and cytochrome f and the activity of a number of enzymes with well-established sites of synthesis. The results confirmed that the higher temperature had induced a specific inhibition of protein synthesis in the plastids. The activity and level of protochlorophyllide reductase was unaffected by growth at the higher temperature, suggesting it to be a cytoplasmically synthesized enzyme.


1 Supported by the Science and Engineering Research Council.







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Copyright © 1982 by the American Society of Plant Biologists