Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 70:1060-1065 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (28)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zilinskas, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zilinskas, B. A.
Agricola
Right arrow Articles by Zilinskas, B. A.
Articles

Isolation and Characterization of the Central Component of the Phycobilisome Core of Nostoc sp. 1,2

Barbara A. Zilinskas

Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, New Jersey 08903

Freshly isolated allophycocyanin is recovered from linear sucrose gradients made in 0.75 molar potassium phosphate buffer (pH 7.0) in three sizes: 19s, 10.3s, and 5.5s. The largest aggregate is a complex of a 680 nm fluorescing allophycocyanin I in the form ({alpha}beta)3{gamma}, where {gamma} is the 95 kilodalton (kD) polypeptide, and two 660 nanometer fluorescing allophycocyanin II ({alpha}beta)3 molecules; the complex, stabilized in high phosphate concentrations, fluoresces maximally at 675 nanometers. The 10.3s fraction is a hexamer of allophycocyanin of the 660 nanometer fluorescing type, perhaps attached through two polypeptides of 46 kD and 44 kD. The 5.5s component of the allophycocyanin pool is the usual trimeric form of allophycocyanin ({alpha}beta)3. A similar 19s fraction is the major component of allophycocyanin I isolated under optimum conditions in the presence of the protease inhibitor, phenylmethylsulfonylfluoride. This 19s fraction is apparently a central component of the core of the phycobilisome with its 95 kD polypeptide the attachment point of the phycobilisome and membrane. The 95 kD polypeptide has both long wavelength absorption and fluorescence bands which seem to account for the long wavelength fluorescence properties of allophycocyanin I.

1 Supported in part by the Science and Education Administration of the United States Department of Agriculture under Grant No. 5901-0410-8-0185-0 from the Competitive Research Grants Office, by a Busch Research Grant and a Rutgers University Research Council Grant.

2 New Jersey Agricultural Experiment Station, Publication No. D-01104-2-82, supported by State Funds and by the United States Hatch Act.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists