Plant Physiol.
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Plant Physiology 70:1107-1114 (1982)
© 1982 American Society of Plant Biologists

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Articles

High Rates of Protein Synthesis by Isolated Chloroplasts 1

Leonard E. Fish and Andre T. Jagendorf2

Plant Biology Section, Cornell University, Ithaca, New York 14853

Improvements are described in the preparation and in vitro conditions of an intact pea (Pisum sativum Progress No. 9) chloroplast system which provides high efficiency for translation of endogenous messenger RNA, using light as an energy source. High rates result in the incorporation into protein of up to 100 nanomoles tritiated leucine per milligram chlorophyll. These rates suggest extensive reinitiation, and repeated utilization of the messenger RNA that code for thylakoid proteins. Up to 39 radioactive thylakoid peptide bands were detected by fluorography after labeling with tritiated leucine.

2 To whom reprint requests should be sent.

1 Supported in part by Grant 5901-0410-9-0327-0 from the United States Department of Agriculture, Science and Education Administration, Competitive Research Grants Organization program in photosynthesis.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists