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Plant Physiology 70:1233-1235 (1982)
© 1982 American Society of Plant Biologists

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Articles

Loss of Hydrogen from Carbon 5 of D-Glucose during Conversion of D-[5-3H,6-14C]Glucose to L-Ascorbic Acid in Pelargonium crispum (L.) L'Hér 1

Michael Grün, Britta Renstrøm and Frank A. Loewus2

Institute of Biological Chemistry and the Biochemistry/Biophysics Program, Washington State University, Pullman, Washington 99164-6340

Conversion of D-[5-3H,6-14C]glucose to L-ascorbic acid in detached apices of Pelargonium crispum (L.) L'Hér cv Prince Rupert (lemon geranium) was accompanied by complete loss of tritium in the product. Chemical degradation of D-glucose which was recovered from the labeled apices yielded D-glyceric acid (corresponding to carbons 4, 5, and 6 of glucose) with a 3H:14C ratio of 4 to be compared with 9, the ratio in D-[5-3H,6-14C]glucose initially. Conversion of D-[6-3H,6-14C]glucose in the same tissue was accompanied by retention of tritium in L-ascorbic acid with a 3H:14C ratio comparable to that of compounds from the hexose pool. Results indicate that during L-ascorbic acid biosynthesis from glucose in Pelargonium crispum hydrogen at carbon 5 undergoes exchange with the medium, suggesting an epimerization at this carbon atom.

2 To whom inquiries and requests for reprints may be addressed.

1 Supported by Grants GM-22427 from the National Institutes of Health and PCM-7813254 from the National Science Foundation. Travel grants were provided by the Deutsche Forschungsgemeinschaft (M. G.) and the Norwegian Research Council of Science and the Humanities (B. R.). Scientific Paper No. 6244, Project 0266, College of Agriculture Research Center, Washington State University, Pullman, WA 99164.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists