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Plant Physiology 70:1425-1428 (1982)
© 1982 American Society of Plant Biologists

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Articles

Characteristics of Membrane-Bound Lectin in Developing Phaseolus vulgaris Cotyledons 1

Maarten J. Chrispeels and Roberto Bollini

Department of Biology C-016, University of California, San Diego, La Jolla, California 92093, Istituto Biosintesi Vegetali, Consiglio Nazionale delle Ricerche, Via Bassini, 15, I-20133, Milan, Italy

Cotyledons of developing Phaseolus vulgaris L. cv Greensleeves seeds were labeled for 2 to 3 hours with 3H-amino acids, and newly synthesized phytohemagglutinin (PHA) was isolated by affinity chromatography with thyroglobulin-Sepharose. The presence of 1% Tween in the homogenate increased the yield of radioactive PHA by 50 to 100%. Isopycnic sucrose gradients were used to show that this detergent-released PHA was associated with the endoplasmic reticulum (ER), and pulse-chase experiments showed that the half-life of the PHA in the ER was 90 to 120 minutes. Since PHA is transiently associated with the ER and accumulates in protein bodies, we postulate that this rapidly turning over pool of PHA in the ER represents protein en route to the protein bodies. The PHA in the ER has the same sedimentation constant as mature PHA and is capable of agglutinating red blood cells. The observations substantiate earlier claims that plant cells contain membrane-bound lectins. However, they also indicate that these lectins are not necessarily functional components of the membranes with which they are associated, but may represent transport pools of lectin normally localized in other cellular compartments.

1 Supported by a grant from the National Science Foundation and North Atlantic Treaty Organization Research Grant 275.81.






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Copyright © 1982 by the American Society of Plant Biologists