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Plant Physiology 70:1673-1677 (1982)
© 1982 American Society of Plant Biologists

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Articles

Non-Mendelian Inheritance of 3-(3,4-Dichlorophenyl)-1,1-dimethylurea-Resistant Thylakoid Membrane Properties in Chlamydomonas1

Ruth E. Galloway2 and Laurens Mets3

Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106

A uniparentally inherited 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)-resistant mutant of Chlamydomonas reinhardii, Dr2, which has a resistance mechanism of the type defined as `primary,' has been isolated. In vitro Hill reactions catalyzed by isolated thylakoid membranes reveal a reduced apparent affinity of the thylakoids for DCMU. These changes in membrane properties quantitatively account for the resistance of mutant Dr2 to herbicide inhibition of growth. The properties of this mutant show that all of the Hill reaction-inhibiting DCMU binding sites are under identical genetic control. Mutant Dr2 is a useful new uniparental genetic marker, since it has a novel phenotype and it may be possible to identify its altered gene product. The low cross-resistance of Dr2 to atrazine suggests that there may be considerable flexibility in exploiting induced herbicide resistance of crop plants for improving herbicide specificity.

Four mendelian mutants in at least three loci all have resistance mechanisms in the class we define as `secondary.' They are as sensitive as wild type to in vitro inhibition of the Hill reaction, and must acquire resistance in vivo by preventing the active form of the herbicide from reaching the sensitive site.

2 Present address: Department of Biology, Washington University, St. Louis, MO 63130.

3 To whom correspondence should be addressed. Present address: Department of Biology, University of Chicago, 1103 E. 57th Street, Chicago, IL 60637.

1 Supported by National Research Service Award Training Grant 5T 32 GM 07225 and National Science Foundation Grants PCM 78-20684 and PCM 80-22722. L. M. is a Fellow of the Andrew Mellon Foundation.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists