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Plant Physiology 71:173-176 (1983)
© 1983 American Society of Plant Biologists

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Articles

In Vivo Synthesis and Turnover of {alpha}-Amylase in Attached and Detached Cotyledons of Vigna mungo Seeds 1

Tomokazu Koshiba and Takao Minamikawa

Department of Biology, Tokyo Metropolitan University, Setagaya-ku, Tokyo 158, Japan

{alpha}-Amylase activity increased in attached cotyledons of germinated Vigna mungo seeds until the 5th day after imbibition and decreased thereafter, whereas in detached and incubated cotyledons the activity continuously increased and, at the 6th day, reached the value more than three times that of the maximum activity of attached cotyledons. Zymograms of the activities and Ouchterlony double immunodiffusion test on the activities of attached and detached cotyledons showed that the increase of activity in detached cotyledons was due to the identical enzyme as in attached tissues. {alpha}-Amylase contents, determined by single radial immunodiffusion method, changed in parallel with enzyme activity in both attached and detached cotyledons, which also suggested the de novo synthesis of {alpha}-amylase in V. mungo cotyledons.

The rate of incorporation of the label from [3H]leucine into {alpha}-amylase and the ratios of dpm in {alpha}-amylase/dpm in trichloroacetic acid-insoluble fraction did not show significant difference between attached and detached cotyledons. The results indicated that in attached cotyledons fluctuation of {alpha}-amylase activity was regulated by both synthesis and degradation of the enzyme, whereas in detached cotyledons {alpha}-amylase was synthesized and accumulated, because of low degrading activity during incubation.

1 Supported partly by Grants-in-Aid (No. 554214 and 56740283) from the Ministry of Education, Science and Culture of Japan, and from Ito Science Foundation (1981).






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Copyright © 1983 by the American Society of Plant Biologists