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Inhibition of -1,4-Glucan Biosynthesis by Deoxyglucose ¹

The Effect on the Glucosylation of Lipid Intermediates

Institüt fur Virologie, Fachbereich Veterinärmedizin, Justus-Liebig-Universität Giessen, D-6300 Giessen, Federal Republic of Germany, Instituto de Investigaciones Biológicas, FCEyN, Universidad Nacional de Mar del Plata and Centro de Investigaciones Biológicas, FIBA, 7600 Mar del Plata, Argentina

GDP- and UDP-deoxyglucose inhibit the incorporation of glucose from UDP-glucose into dolichyl phosphate glucose and dolichyl pyrophosphate oligosaccharides. GDP-deoxyglucose inhibits by competing with the physiological nucleotide sugars for dolichyl phosphate, and dolichyl phosphate deoxyglucose is formed. This inhibition is reversed by excess of dolichyl phosphate. UDP-deoxyglucose does not give rise to a lipid-linked derivative, and inhibition by this analog is not reversed by dolichyl phosphate. The UDP- and GDP-derivatives of deoxyglucose inhibit the incorporation of glucose into glucose-containing glycoproteins. This effect seems to be the result of the inhibition of lipid intermediates glucosylation and is comparable to the effect produced by coumarin. Cellulose synthetase activity is not affected by UDP- or GDP-deoxyglucose. On the other hand, deoxyglucose inhibits the formation of -1,4-glucans in vivo.