This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wilson, K. A. Articles by Chen, J. C. Search for Related Content PubMed PubMed Citation Articles by Wilson, K. A. Articles by Chen, J. C. Agricola Articles by Wilson, K. A. Articles by Chen, J. C.

Articles

Amino Acid Sequence of Mung Bean Trypsin Inhibitor and Its Modified Forms Appearing during Germination ¹

Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13901

The amino acid sequence of the major trypsin inhibitor, F, of ungerminated mung beans (Vigna radiata [L.] Wilczek) was determined by a combination of automatic solid phase and manual sequencing techniques. F is a typical Bowman-Birk-type proteinase inhibitor with 80 amino acid residues and exhibits a high degree of identity with the other sequenced members of the Bowman-Birk family of inhibitors. Thin layer peptide maps of mung bean inhibitors E and C (which appear during germination) indicate that both are derived from inhibitor F by limited specific proteolysis. Loss of the carboxyl-terminal residues 77 to 80 from F produces inhibitor E, while the loss of an additional two carboxyl-terminal residues, the loss of the amino-terminal residues 1 to 8, and an internal cleavage at Ala³⁵-Asp³⁶ produces inhibitor C from E. Another inhibitor species, E', was isolated from ungerminated seeds. It differs from F in the loss of residues 1 to 6. The majority of the proteolytic cleavages noted in the F-E-C-E' system are at peptide bonds involving aspartyl residues.

¹ Supported by National Science Foundation Grant PCM 8003854. In memory of the late Dr. Michael Laskowski, Sr.

This article has been cited by other articles:

				R. C. L. Teles, L. d. A. Calderon, F. J. Medrano, J. A. R. G. Barbosa, B. G. Guimaraes, M. M. Santoro, and S. M. de Freitas pH Dependence Thermal Stability of a Chymotrypsin Inhibitor from Schizolobium parahyba Seeds Biophys. J., May 1, 2005; 88(5): 3509 - 3517. [Abstract] [Full Text] [PDF]

				P. Kumar, A. G. A. Rao, S. Hariharaputran, N. Chandra, and L. R. Gowda Molecular Mechanism of Dimerization of Bowman-Birk Inhibitors: PIVOTAL ROLE OF ASP76 IN THE DIMERZATION J. Biol. Chem., July 16, 2004; 279(29): 30425 - 30432. [Abstract] [Full Text] [PDF]