Plant Physiol. Drug Metab Dispos
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Plant Physiology 71:379-387 (1983)
© 1983 American Society of Plant Biologists

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Articles

Role of the Colorless Polypeptides in Phycobilisome Assembly in Nostoc sp. 1

Barbara A. Zilinskas and Dawn A. Howell

Department of Biochemistry and Microbiology, New Jersey Agricultural Experiment Station, Cook College, Rutgers University, New Brunswick, New Jersey 08903

We have identified the function of the `extra' polypeptides involved in phycobilisome assembly in Nostoc sp. These phycobilisomes, as those of other cyanobacteria, are composed of an allophycocyanin core, phycoerythrin- and phycocyanin-containing rods, and five additional polypeptides of 95, 34.5, 34, 32, and 29 kilodaltons. The 95 kilodalton polypeptide anchors the phycobilisome to the thylakoid membrane (Rusckowski, Zilinskas 1982 Plant Physiol 70: 1055-1059); the 29 kilodalton polypeptide attaches the phycoerythrin- and phycocyanin-containing rods to the allophycocyanin core (Glick, Zilinskas 1982 Plant Physiol 69: 991-997). Two populations of rods can exist simultaneously or separately in phycobilisomes, depending upon illumination conditions. In white light, only one type of rod with phycoerythrin and phycocyanin in a 2:1 molar ratio is synthesized. Associated with this rod are the 29, 32, and 34 kilodalton colorless polypeptides; the 32 kilodalton polypeptide links the two phycoerythrin hexamers, and the 34 kilodalton polypeptide attaches a phycoerythrin hexamer to a phycocyanin hexamer. The second rod, containing predominantly phycocyanin, and the 34.5 and 29 kilodalton polypeptides, is synthesized by redlight-adapted cells; the 34.5 kilodalton polypeptide links two phycocyanin hexamers. These assignments are based on isolation of rods, dissociation of these rods into their component biliproteins, and analysis of colorless polypeptide composition, followed by investigation of complexes formed or not formed upon their recombination.

1 Supported in part by the Science and Education Administration of the United States Department of Agriculture under Grant 5901-0410-8-0185-0 from the Competitive Research Grants Office, by a National Science Foundation Grant PCM-80-18740, by a Busch Research Grant, and by a Rutgers University Research Council Grant. New Jersey Agricultural Experiment Station, Publication No. D-01104-3-82, supported by State funds and by the United States Hatch Act.






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Copyright © 1983 by the American Society of Plant Biologists