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Plant Physiology 71:447-450 (1983)
© 1983 American Society of Plant Biologists

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Articles

Comparison of the Glyoxysomes and the Glyoxysomal Enzymes in Maize Lines with High or Low Oil Content 1

Athanasios S. Tsaftaris2 and John G. Scandalios3

Department of Genetics, North Carolina State University, Raleigh, North Carolina 27650

The developmental profile of the glyoxysomes and their component enzymes catalase, malate synthase, and isocitrate lyase were compared in the scutellum of two maize (Zea mays) lines, Illinois High Oil (IHO, approximately 20% lipid content) and Illinois Low Oil (ILO, less than 0.5% lipid content). The microbodies participate in the catabolism of the seed lipids and are responsible for leading the catabolic products (acetyl-Coenzyme A) into gluconeogenesis. The aim of this study was to determine whether changes in lipid content of the seed resulted in changes in the levels of the glyoxysomal enzymes. Enzyme activity measurements, immunological measurements (in the case of catalase), cell fractionation studies, and electron microscopic observations indicated that the IHO and ILO lines contain similar populations of glyoxysomes and exhibit similar catalase and malate synthase specific activities, despite the significant difference (40-fold) in their lipid content. Only the specific activity of isocitrate lyase was higher (2-fold higher) in the IHO seeds as compared to the ILO.

2 Present address: Department of Genetics and Plant Breeding, University of Thessaloniki, Thessaloniki, Greece.

3 To whom requests for reprints should be addressed.

1 Supported by National Institutes of Health Research Grant GM 22733-05 to J. G. S. Paper No. 8446 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists