Plant Physiol.
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Plant Physiology 71:547-550 (1983)
© 1983 American Society of Plant Biologists

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Articles

Characterization of a Selenocystine-Resistant Carrot Cell Line 1

Alterations in Cystine and Sulfate Uptake

Ian J. Furner2 and Zinmay R. Sung

Department of Genetics, University of California, Berkeley, California 94720, Department of Plant Pathology, University of California, Berkeley, California 94720

A selenocystine-resistant carrot cell line, C-1, was isolated from a haploid carrot (Daucus carota) cell culture, HA. The C-1 variant takes up cystine, but not cysteine, more slowly than does HA. The selenocystine resistance is maintained in culture in the absence of selection and is expressed in regenerated plants. Results based on chromatographic separation of sulfur metabolites from cells fed with [35S]cystine suggest a block either in the uptake or reduction of cystine in the variant. Both lines can grow on cystine as sole sulfur source. Growth of the HA line on cystine suppressed the development of sulfate uptake capacity (Furner, Sung 1982 Proc Natl Acad Sci USA 79: 1149-1153), while cystine-grown C-1 cells have high levels of sulfate uptake capacity.

We suggest that the C-1 line, grown on cystine, accumulates an insufficient quantity of some sulfur metabolite, which is involved in the control of sulfate uptake, to suppress the uptake. C-1 grown on cystine is more sensitive than HA to growth inhibition by the sulfate analog selenate.

2 Supported in part by a fellowship from the ARCO Plant Cell Research Institute. Present address: Department of Microbiology and Immunology, University of Washington, Seattle, WA 98195.

1 Supported by United States Department of Agriculture Competitive Grant AG5901-0410-9-0370-0, and National Science Foundation Grant PCM-8021209.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists