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Articles

Changes in Tobacco Cell Membrane Composition and Structure Caused by Cercosporin ¹

Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824, Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

Cercosporin, a toxin produced by Cercospora species, rapidly kills plant cells in the light. Previous work has shown that cercosporin treatment causes products of lipid peroxidation to be released. We have found that the unsaturated acyl chains of lipids in tobacco (Nicotiana tabacum) cell membranes are destroyed when cells are treated with cercosporin. Concomitant with this change in composition is a change in structure of the membranes as detected by two different fatty acid spin labels, 2-(3-carboxypropyl)-4,4-dimethyl-2-tridecyl-3-oxazolidinyloxyl (denoted I[12,3]) and 2-(14-carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl (denoted I[1,14]). Cercosporin causes the membranes to become more rigid at all temperatures tested and increases the membrane phase transformation temperature from 12.7°C to 20.8°C.

³ Present address: Department of Biotechnology Research, Pioneer Hi-Bred International, Inc., Box 38, Johnston, IA 50131.

¹ Supported in part by United States Department of Agriculture Competitive Grant No. 159-2261-0-1-420-0 and by a Michigan Agricultural Experiment Station Graduate Research Assistantship (S.P.B.). Michigan Agricultural Experiment Station Journal Article No. 10595.

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