Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 71:780-784 (1983)
© 1983 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (93)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wallsgrove, R. M.
Right arrow Articles by Miflin, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wallsgrove, R. M.
Right arrow Articles by Miflin, B. J.
Agricola
Right arrow Articles by Wallsgrove, R. M.
Right arrow Articles by Miflin, B. J.
Articles

Intracellular Localization of Aspartate Kinase and the Enzymes of Threonine and Methionine Biosynthesis in Green Leaves

Roger M. Wallsgrove, Peter J. Lea and Benjamin J. Miflin

Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Hertfordshire, United Kingdom

The intracellular localization of several aspartate pathway enzymes has been studied in pea (Pisum sativum cv Feltham First) and barley (Hordeum vulgare cv Julia) leaves. Protoplast lysates were fractionated by differential or sucrose density gradient centrifugation, in media optimized for each enzyme. The results show that aspartate kinase, homoserine kinase, threonine synthase, and cystathionine {gamma}-synthase are confined to the chloroplast. Cystathionine {beta}-lyase appears to be present in several fractions, though more than 50% of the total activity is associated with the chloroplasts. In contrast, neither methionine synthase nor methionine adenosyl-transferase were significantly associated with chloroplasts, and only a small proportion of the methionine synthase was associated with the mitochondrial fraction. Methionine adenosyltransferase, and hence S-adenosylmethionine synthesis, is not found in any organelle fraction. The conclusion is that whereas threonine, like lysine, is synthesized only in the chloroplast, the last step in methionine biosynthesis occurs largely in the cytoplasm.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
C. Ibar and A. Orellana
The Import of S-Adenosylmethionine into the Golgi Apparatus Is Required for the Methylation of Homogalacturonan
Plant Physiology, October 1, 2007; 145(2): 504 - 512.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. Okazaki, M. Sugawara, K.-I. Yuhashi, and K. Minamisawa
Rhizobitoxine-induced Chlorosis Occurs in Coincidence with Methionine Deficiency in Soybeans
Ann. Bot., July 1, 2007; 100(1): 55 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Bouvier, N. Linka, J.-C. Isner, J. Mutterer, A. P.M. Weber, and B. Camara
Arabidopsis SAMT1 Defines a Plastid Transporter Regulating Plastid Biogenesis and Plant Development
PLANT CELL, November 1, 2006; 18(11): 3088 - 3105.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Palmieri, R. Arrigoni, E. Blanco, F. Carrari, M. I. Zanor, C. Studart-Guimaraes, A. R. Fernie, and F. Palmieri
Molecular Identification of an Arabidopsis S-Adenosylmethionine Transporter. Analysis of Organ Distribution, Bacterial Expression, Reconstitution into Liposomes, and Functional Characterization
Plant Physiology, November 1, 2006; 142(3): 855 - 865.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. Hesse, O. Kreft, S. Maimann, M. Zeh, and R. Hoefgen
Current understanding of the regulation of methionine biosynthesis in plants
J. Exp. Bot., August 1, 2004; 55(404): 1799 - 1808.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ravanel, M. A. Block, P. Rippert, S. Jabrin, G. Curien, F. Rebeille, and R. Douce
Methionine Metabolism in Plants: CHLOROPLASTS ARE AUTONOMOUS FOR DE NOVO METHIONINE SYNTHESIS AND CAN IMPORT S-ADENOSYLMETHIONINE FROM THE CYTOSOL
J. Biol. Chem., May 21, 2004; 279(21): 22548 - 22557.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Kreft, R. Hoefgen, and H. Hesse
Functional Analysis of Cystathionine gamma -Synthase in Genetically Engineered Potato Plants
Plant Physiology, April 1, 2003; 131(4): 1843 - 1854.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Zeh, A. P. Casazza, O. Kreft, U. Roessner, K. Bieberich, L. Willmitzer, R. Hoefgen, and H. Hesse
Antisense Inhibition of Threonine Synthase Leads to High Methionine Content in Transgenic Potato Plants
Plant Physiology, November 1, 2001; 127(3): 792 - 802.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Chiba, M. Ishikawa, F. Kijima, R. H. Tyson, J. Kim, A. Yamamoto, E. Nambara, T. Leustek, R. M. Wallsgrove, and S. Naito
Evidence for Autoregulation of Cystathionine -Synthase mRNA Stability in Arabidopsis
Science, November 12, 1999; 286(5443): 1371 - 1374.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Ravanel, B. Gakiere, D. Job, and R. Douce
The specific features of methionine biosynthesis and metabolism in plants
PNAS, June 23, 1998; 95(13): 7805 - 7812.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Aubert, G. Curien, R. Bligny, E. Gout, and R. Douce
Transport, Compartmentation, and Metabolism of Homoserine in Higher Plant Cells . Carbon-13- and Phosphorus-31-Nuclear Magnetic Resonance Studies
Plant Physiology, February 1, 1998; 116(2): 547 - 557.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists