Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 71:869-873 (1983)
© 1983 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (59)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hanks, J. F.
Right arrow Articles by Tolbert, N. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hanks, J. F.
Right arrow Articles by Tolbert, N. E.
Agricola
Right arrow Articles by Hanks, J. F.
Right arrow Articles by Tolbert, N. E.
Articles

Isolation and Characterization of Infected and Uninfected Cells from Soybean Nodules 1

Role of Uninfected Cells in Ureide Synthesis

Joanna F. Hanks, Karel Schubert and N. E. Tolbert

Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824

The distribution of organelles and associated enzymes between cells containing bacteroids and uninfected cells from nodules of Glycine max L. Merr. cv Amsoy 71 was investigated by separation of protoplasts on a sucrose step-gradient. Infected protoplasts were much larger, irregular in shape, and more dense than uninfected protoplasts. The peroxisomal enzymes, uricase and catalase, were present at much higher specific activity in the uninfected cell fraction. Allantoinase, an enzyme of the endoplasmic reticulum, had a greater specific activity in the uninfected cell fraction. Several enzymes whose products are required for purine biosynthesis, including phosphoglycerate dehydrogenase, aspartate aminotransferase, 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase, exhibited a higher specific activity in the uninfected cell fraction. Isozymes of aspartate aminotransferase were separated on native gels and located by an activity stain. The soluble isozyme was predominantly found in the uninfected cell fraction. These data suggest that peroxisomes, containing uricase and catalase for conversion of uric acid to allantoin, are present only in the uninfected cells of soybean nodules. The uninfected cells also appear to be the site of the allantoinase reaction.

1 Supported in part by a grant from the National Science Foundation to N. E. T. (PCM 78 15891). J. F. H. vas supported by a Graduate Professional Opportunity Program Fellowship from the National Institutes of Health. Published as journal article 10540 of the Michigan Agricultural Experiment Station. A preliminary report has been published (9).




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Raychaudhuri and P. A. Tipton
Cloning and Expression of the Gene for Soybean Hydroxyisourate Hydrolase. Localization and Implications for Function and Mechanism
Plant Physiology, December 1, 2002; 130(4): 2061 - 2068.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists