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Plant Physiology 71:912-915 (1983)
© 1983 American Society of Plant Biologists

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Articles

Inhibition of Glutamine Synthetase from Pea by Tabtoxinine-{beta}-lactam

Michael D. Thomas, Pat J. Langston-Unkefer, Thomas F. Uchytil and Richard D. Durbin

Department of Plant Pathology, University of Wisconsin—Madison and United States Department of Agriculture, Agricultural Research Service, Department of Agronomy, University of Wisconsin—Madison and United States Department of Agriculture, Agricultural Research Service

Tabtoxinine-{beta}-lactam, a hydrolytic product of tabtoxin produced by Pseudomonas syringae pv. tabaci, apparently inactivates pea seed glutamine synthetase. Inhibition of the enzyme's initial velocity is linear over a range of 0.5 to 5 millimolar tabtoxinine-{beta}-lactam in the presence of 10 millimolar glutamate. A method for the purification of glutamine synthetase from dried peas is presented which gives a 30% yield with a 2,000-fold increase in specific activity. A method for obtaining highly purified tabtoxinine-{beta}-lactam and tabtoxin in good yields is also presented. The authenticity and purity of tabtoxinine-{beta}-lactam and tabtoxin were verified by chromatography, biological activity, and 1H and 13C nuclear magnetic resonance spectroscopy.




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C. L. Bender, F. Alarcon-Chaidez, and D. C. Gross
Pseudomonas syringae Phytotoxins: Mode of Action, Regulation, and Biosynthesis by Peptide and Polyketide Synthetases
Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 266 - 292.
[Abstract] [Full Text] [PDF]


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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists