Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 71:927-931 (1983)
© 1983 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moloney, M. M.
Right arrow Articles by Elliott, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moloney, M. M.
Right arrow Articles by Elliott, M. C.
Agricola
Right arrow Articles by Moloney, M. M.
Right arrow Articles by Elliott, M. C.
Articles

Auxin Requirements of Sycamore Cells in Suspension Culture

Maurice M. Moloney1, John F. Hall, Gillian M. Robinson and Malcolm C. Elliott

School of Life Sciences, Leicester Polytechnic, Leicester LE1 9BH United Kingdom

Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this `auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic acid (IAA). Changes in free 2,4-D and IAA in the cells during a culture passage have been monitored.

There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 nanograms per 106 cells) on day 2 followed by a decline to 45 nanograms per 106 cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 nanograms per 106 cells) rises slowly to a peak of 1.4 nanograms per 106 cells by day 9 then decreases rapidly to 0.2 nanograms per 106 cells by day 15 (early declining phase) and 0.08 nanograms per 106 cells by day 23 (early stationary phase).

1 Present address: Institut de Biologie et de Physiologie Végétales, Université de Lausanne, 1005 Lausanne, Switzerland.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists