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Plant Physiology 72:177-181 (1983)
© 1983 American Society of Plant Biologists

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Computer-Assisted Image Analysis of Tissues of Ethrel-Treated Pinus taeda Seedlings 1

Frank W. Telewski, Andrew H. Wakefield and Mordecai J. Jaffe

Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109

A microcomputer based video image analyzer is described and demonstrated in the analysis and comparison of tissue types of 6-month-old Ethrel-treated and control seedlings of loblolly pine (Pinus taeda L.). Two different programs were employed. `Topographer I' sought, identified, and measured cell or resin canal lumen areas, and `Area' measured tracheid length and silhouette areas. Ethrel treatment induced an increase in radial growth and a decrease in shoot elongation and needle growth. Bark production was stimulated by the same treatment. The tracheid lumens of Ethrel-treated plants were shorter and had a smaller cross-sectional area, whereas the tracheid cell walls increased in thickness. The treatment also increased resin canal formation in the xylem.


1 Supported by National Science Foundation grant PCM 77/24798 and National Aeronautics and Space Administration grant NAGW-96 to M. J. J.




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Copyright © 1983 by the American Society of Plant Biologists