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Plant Physiology 72:200-203 (1983) © 1983 American Society of Plant Biologists 5-Aminolevulinate Synthesis in Permeabilized Filaments of the Blue-Green Alga Anabaena variabilisDepartment of Applied Hydrobiology, Desert Research Institute, Ben Gurion University of the Negev, Beer Sheva, 84120, Israel, Department of Biology, Ben Gurion University of the Negev, Beer Sheva, 84120, Israel
Filaments of the blue-green alga Anabaena variabilis permeabilized by dimethylsulfoxide (DMSO) produce increased amounts of 5-aminolevulinate in the presence of levulinic acid. The metabolic activity of the filaments remains unperturbed in the presence of up to 7.5% (v/v) DMSO. Studies utilizing DMSO-permeabilized filaments confirm that 5-aminolevulinate is synthesized preferably from glutamate and, to a lesser extent, from
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