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Plant Physiology 72:60-65 (1983)
© 1983 American Society of Plant Biologists

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Articles

A Guide to Establishing Water Potential of Aqueous Two-Phase Solutions (Polyethylene Glycol plus Dextran) by Amendment with Mannitol 1

Burlyn E. Michel, Olson K. Wiggins and William H. Outlaw, Jr.

Department of Botany, University of Georgia, Athens, Georgia 30602, Department of Biological Science (Unit 1), Florida State University, Tallahassee, Florida 32306

A practical guide to calculating the mannitol (MAN) amendment required to achieve the desired water potential ({Psi}) of polyethylene glycol/dextran (PEG/DEX) aqueous two-phase systems for protoplast purification is presented. The empirically generated equation {Psi} = 305[PEG']2[MAN] + 0.74[PEG'][MAN]T – 103[PEG'][MAN] + 5.6[PEG']2T – 623[PEG']2 – 0.25[PEG']T + 12.7[PEG'] – 0.078[MAN]T – 22.75[MAN]accurately predicts experimental {Psi} (in bars). [PEG'] indicates the presence of DEX where [DEX] = [PEG]/(0.6–0.4[PEG]). The equation is applicable for these ranges: [PEG'] from 0.047 to 0.13 gram per gram H2O; [MAN] from 0 to 0.7 molal; T from 4.5 to 40°C. Actual {Psi} should differ from derived {Psi} by no more than 8% for the least negative values to 4% for the most negative values. The {Psi} for solutions of MAN, of PEG, and of DEX were also determined. Equations to fit data for each were generated. Analyses indicated a significant synergistic effect on {Psi} when MAN is added to PEG/DEX and, at certain concentrations, between PEG and DEX.

1 Supported by a grant to W. H. O. from the United States Department of Energy.




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S. Q. Zhang, W. H. Outlaw, and K. Aghoram
Relationship between changes in the guard cell abscisic-acid content and other stress-related physiological parameters in intact plants
J. Exp. Bot., February 1, 2001; 52(355): 301 - 308.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Society of Plant Biologists