Plant Physiol. Drug Metab Dispos
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Plant Physiology 72:273-279 (1983)
© 1983 American Society of Plant Biologists

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Articles

Similarities and Differences in Lipid Metabolism of Chloroplasts Isolated from 18:3 and 16:3 Plants

Ernst Heinz1 and P. Grattan Roughan

Plant Physiology Division, Department of Scientific and Industrial Research, Private Bag, Palmerston North, New Zealand

Photosynthetically active chloroplasts retaining high rates of fatty acid synthesis from [1-14C]acetate were purified from leaves of both 16:3 (Solanum nodiflorum, Chenopodium album) and 18:3 plants (Amaranthus lividus, Pisum sativum). A comparison of lipids into which newly synthesized fatty acids were incorporated revealed that, in 18:3 chloroplasts, enzymic activities catalyzing the conversion of phosphatidate to diacylglycerol and of diacylglycerol to monogalactosyl diacylglycerol (MGD) were significantly less active than in 16:3 chloroplasts. In contrast, labeling rates of MGD from UDP-[14C]gal were similar for both types of chloroplasts.

The composition and positional distribution of labeled fatty acids within the glycerides synthesized by isolated 16:3 and 18:3 chloroplasts were similar and in each case only a C18/C16 diacylglycerol backbone was synthesized. In nodiflorum chloroplasts, C18:1/C16:0 MGD assembled de novo was completely desaturated to the C18:3/C16:3 stage.

Whereas newly synthesized C18/C18 MGD could not be detected in any of these chloroplasts if incubated with [14C]acetate after isolation, chloroplasts isolated from acetate-labeled leaves contained MGD with labeled C18 fatty acids at both sn-1 and sn-2 positions. Taken together, these results provide further evidence on an organellar level for the operation of pro- and eucaryotic pathways in the biosynthesis of MGD in different groups of plants.

1 On leave from the Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, 5000 Köln 41, Federal Republic of Germany, and supported by a travel grant from the Deutsche Forschungsgemeinschaft. Reprint requests should be directed to this address.




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