Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 72:394-397 (1983)
© 1983 American Society of Plant Biologists

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Articles

Early Growth of Wheat Embryonic Axes and the Synthesis of RNA and DNA 1

Ketaki Datta, Loren Marsh and Abraham Marcus2

Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111

The requirement for the synthesis of RNA and DNA in early germination of wheat (Triticum aestivum var Newana) embryonic axes has been studied by incubating embryos in the presence of appropriate inhibitors and monitoring both embryo growth and the rates of specific metabolic processes. Experiments with 5-fluorouridine showed that both rRNA and DNA synthesis could be curtailed by 60 to 70% without affecting embryo growth to 24 hours. Similarly, the presence of mitomycin C and methotrexate inhibited DNA synthesis 70%, with only a small effect on growth. Experiments with a range of concentrations of cordycepin and {alpha}-amanitin indicated that mRNA synthesis could be curtailed by 30 to 40% within the first 8 hours of germination with only a small effect on embryo growth. Thus, at least the initial phases of seed embryo germination are not closely linked to the synthesis of mRNA, rRNA, or DNA. Maximal sensitivity of embryo growth was obtained with cycloheximide and 2-(4-methyl-2,6-dinitroanilino)-N-methyl propionamide, supporting the idea that protein synthesis is the macromolecular process most closely linked to early germination.

2 To whom reprint requests should be sent.

1 Supported by grant PCM81-04538 from the National Science Foundation, by United States Public Health Service grants CA-06927 and RR-05539 from the National Institutes of Health, and by an appropriation from the Commonwealth of Pennsylvania.






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